Effect of proteolytic and lipolytic Enzymes on the Electron Transport Particle Fraction of Rhodospirillum rubrum
نویسنده
چکیده
Treatment of the electron transport membrane fraction of Rhodospirillum rubrum with phospholipase A from the venom of Naja Naja resulted in a stimulation of NADHand succinate-cytochrome c-reductase of the electron transfer chain. The mechanism of this stimulation appears to be identical with that found in the presence of the anionic detergent deoxycholate. At the same time the NADH oxidase system is inactivated while NADHand succinate dehydrogenase remain unaffected. None of the activities mentioned becomes solubilized as the result of phospholipase action; likewise no protein is released. The stimulated activities, the kinetic properties of which remain unchanged, are inactivated by proteases at significantly slower rates than those of particles not treated with phospholipase A. The phospholipases C and D were found to be without any effect on the membranes. The following additional information was obtained with phospholipase A in the system studied here: The enzyme from Naja Naja venom does not require Ca2® for activity. Phospholipase A prepared from the venom of Crotalus adamanteus is completely ineffective with the electron transfer activities of the particulate fraction.
منابع مشابه
Effect of proteolytic and lipolytic enzymes on the electron transport particle fraction of Rhodospirillum rubrum. II. Phospholipases.
Digestion of the electron transport particle fraction of Rhodospirillum rubrum with the proteases chymotrypsin, trypsin, subtilisin and pronase resulted in a release of protein from the membranal system. The solubilization was, however, limited to only 15 — 18 percent of the total protein, being the same with each of the four proteases. The enzymes catalyzing electron transfer which are located...
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